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VLK Polyclonal Antibody
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FIGURE: 1 / 3
VLK Antibody (600-401-FX0) in ICC/IF
Immunofluorescence Microscopy of VLK antibody. Tissue: Rat lung tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: VLK antibody at 5 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: VLK is cytoplasmic. Staining: VLK as red fluorescent signal.
Product Details
600-401-FX0
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-VLK antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the internal region of human VLK.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
VLK was identified as a novel protein kinase that was induced after the differentiation of cultured embryonic stem cells into mesendoderm. It has no homologs in invertebrates, but is highly conserved in vertebrate species although it does not belong to any known protein kinase groups. VLK is initially expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, but its expression is later confined to E-cadherin-negative mesenchyme. It is enriched in the Golgi apparatus and is thought to regulate the rate of protein export from the Golgi. Targeted disruption of VLK in mice leads to a defect in lung development and neonatal lethality. It has been suggested that mutations in VLK may be associated with the allergic condition atopy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Extracellular tyrosine-protein kinase PKDCC; protein kinase domain containing, cytoplasmic homolog; Protein kinase domain-containing protein, cytoplasmic; Protein kinase-like protein SgK493; Sugen kinase 493; Vertebrate lonesome kinase
Gene Aliases: Adtk1; AI115348; AW548124; ESTM17; MAd1; PKDCC; RGD1311939; RLSDF; SGK493; VLK; X83346
UniProt ID: (Human) Q504Y2, (Mouse) Q5RJI4
Entrez Gene ID: (Human) 91461, (Rat) 313860, (Mouse) 106522
skeletal system development
peptidyl-tyrosine phosphorylation
bone mineralization
positive regulation of bone mineralization
positive regulation of chondrocyte differentiation
limb morphogenesis
multicellular organism growth
negative regulation of Golgi to plasma membrane protein transport
lung alveolus development
embryonic digestive tract development
palate development
ossification
protein transport
cell differentiation
protein phosphorylation
transport
multicellular organism development
phosphorylation
protein autophosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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