Invitrogen

Vimentin Monoclonal Antibody (J144)

Name: Vimentin Monoclonal Antibody (J144)
Brand: Invitrogen
SKU: MA3-745

高级验证

13个已发表图片

24 篇参考文献

查看其他109个Vimentin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Vimentin Antibody in Immunocytochemistry (ICC/IF)

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Vimentin Antibody (MA3-745) in ICC/IF

Immunofluorescent analysis of Vimentin (green) in HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Vimentin mouse monoclonal antibody (Product # MA3-745) at a dilution of 1:100 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 con... View More

Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Vimentin Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Vimentin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Vimentin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Immunogen image

Immunogen Image Vimentin Monoclonal Antibody (J144)

产品信息

MA3-745

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看10篇已发表文章 10篇已发表文章

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

1:20

查看6篇已发表文章 6篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

J144

抗原

Human rhabdosarcoma cell line JR1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_326285

产品详细信息

MA3-745 detects human, rat, and mouse vimentin and has been successfully used in HELA-S3, HEK-293, rat brain, and rat kidney cells.

MA3-745 has been successfully used in Western blot, immunohistochemical and immunofluorescence procedures. By Western blot, this antibody detects an ~57 kDa protein representing vimentin from HELA-S3 cells. Immunohistochemical staining of vimentin using MA3-745 can be performed.

The MA3-745 immunogen was human rhabdosarcoma cell line JR1.

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。