Invitrogen

Vimentin Recombinant Mouse Monoclonal Antibody (V9), Alexa Fluor™ Plus 647

Name: Vimentin Recombinant Mouse Monoclonal Antibody (V9), Alexa Fluor\u2122 Plus 647
Brand: Invitrogen
SKU: 750013MP647

高级验证

查看其他116个Vimentin抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (3)
高级验证 (2)

Vimentin Antibody in Immunocytochemistry (ICC/IF)

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Vimentin Antibody (750013MP647) in ICC/IF

Immunofluorescence analysis of Vimentin was performed using Vimentin Recombinant Mouse Monoclonal Antibody (V9), Alexa Fluor™ Plus 647 (Product # 750013MP647) on 70-80% confluent HeLa and MCF-7 cells. The cell lines were fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.1% Triton X-100 for 15 min and blocked with 2% BSA for 1 hr at room temperature. The cells were stained with Vimentin Recombinant Mouse Monoclonal Antibody (V9), Alexa Fluor™ Plus 647 (Product # 750013MP647, 1:200) in 0.1% BSA, incubated at 4 degree Celsius ov... View More

Vimentin Antibody in Flow Cytometry (Flow)

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产品信息

750013MP647

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

免疫细胞化学 (ICC/IF)

1:200

流式细胞分析 (Flow)

1 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

偶联物

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

激发/发射光谱

658/675 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

50 µg

纯化类型

Protein A/G

保存液

proprietary buffer, pH 6.3-7.3

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。