Invitrogen

Vinculin Recombinant Rabbit Monoclonal Antibody (42H89L44)

Name: Vinculin Recombinant Rabbit Monoclonal Antibody (42H89L44)
Brand: Invitrogen
SKU: 700062

高级验证

29个已发表图片

63 篇参考文献

查看其他54个Vinculin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

图: 1 / 40

Vinculin Antibody (700062) in WB

Knockout of Vinculin was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR948199_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Vinculin was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa Vinculin KO (Lane 3) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Produ... View More

Vinculin Antibody in Immunocytochemistry (ICC/IF)

Vinculin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Vinculin Antibody in Immunoprecipitation (IP)

Vinculin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Vinculin Antibody in Immunohistochemistry (IHC)

Vinculin Antibody in Proximity Ligation Assay (PLA) (PLA)

Product Image Gallery

Immunogen image

Immunogen Image Vinculin Recombinant Rabbit Monoclonal Antibody (42H89L44)

产品信息

700062

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.0025-3 µg/mL

查看34篇已发表文章 34篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

0.5-5 µg/mL

查看21篇已发表文章 21篇已发表文章

流式细胞分析 (Flow)

2-4 µg/1x10^6 cells

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

Proximity Ligation Assay (PLA) (PLA)

查看1篇已发表文章 1篇已发表文章

凝胶阻滞分析 (GS)

查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Deer, Fruit fly, Hamster, Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

42H89L44

抗原

A recombinant protein corresponding to amino acids 408-591 of P18206.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532280

产品详细信息

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

This antibody reacts with Human Vinculin. Based on sequence similarity, reactivity to chimpanzee, Rhesus monkey, swine, equine, mouse, rat, bovine, and chicken is expected. Immunogen sequence IRGALAEARKIAELCDDPKERDDILRSLGEI SALTSKLADLRRQGKGDSPEARALAKQV ATALQNLQTKTNRAVANSRPAKAAVHLE GKIEQAQRWIDNPTVDDRGVGQAAIRGL VAEGHRLANVMMGPYRQDLLAKCDRVD QLTAQLADLAARGEGESPQARALASQLQ DSLKDLKARMQEAM. Test material for western blotting/immunofluorescence: HeLa cells.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

仅用于科研。不用于诊断过程。未经明确授权不得转售。