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Invitrogen
WAPL Polyclonal Antibody
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WAPL Antibody (PA5-143906) in ICC/IF
Immunocytochemistry analysis of WAPL in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in WAPL Polyclonal antibody (Product # PA5-143906) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
产品信息
PA5-143906
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human WAPL/FOE recombinant protein (Position: Q916-C1190).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human 293T whole cell, human HepG2 whole cell, rat L6 whole cell, mouse C2C12 whole cell. IHC: mouse colon tissue, human lymphoma tissue, human stomach cancer tissue, human tonsil tissue. ICC/IF: T-47D cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Transcriptional repressor which may play a role in development of the central nervous system (CNS).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DIF-2; Dioxin-inducible factor 2; friend of EBNA2; friend of EBNA2 (Epstein-Barr virus nuclear protein 2); Friend of EBNA2 protein; WAPL cohesin release factor; wings apart-like homolog (Drosophila); Wings apart-like protein homolog
基因别名: A530089A20Rik; BC037674; DIF-2; FOE; KIAA0261; RGD1308960; WAPAL; WAPL
UniProt ID: (Human) Q7Z5K2, (Mouse) Q65Z40
Entrez Gene ID: (Human) 23063, (Rat) 290577, (Mouse) 218914
mitotic nuclear division
negative regulation of DNA replication
response to toxic substance
negative regulation of chromatin binding
negative regulation of sister chromatid cohesion
positive regulation of fibroblast proliferation
regulation of chromosome segregation
regulation of chromosome condensation
protein localization to chromatin
regulation of cohesin loading
sister chromatid cohesion
mitotic sister chromatid cohesion
viral process
meiotic chromosome segregation
cell division
cell cycle
