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Agrisera
WHIRLY2 Polyclonal Antibody
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WHIRLY2 Antibody (AS16-3952) in IHC (PFA)
Material: Hordeum vulgare (flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White Samples: ultrathin sections on Au-Grids Immunogold labelling: 1. Blocking 30 min RT in PBS (p H 7,4) + 0,2% BSA + 0,2% fish gelatin (= blocking buffer) sterile filtrated 2. Incubation over night at 4°C anti-WHIRLY 2 (chicken antibodies) 1:100 in blocking buffer 3. Washing: 4 x 3 min PBS + 0,1% BSA... View More
产品信息
AS16-3952
产品规格
种属反应
Plant
宿主/亚型
Chicken
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from HvWHIRLY2 protein sequence.
偶联物
形式
Liquid
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 8
内含物
0.02% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Immunogen affinity purified IgY
Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Hordeum vulgare
靶标信息
WHIRLY2 is a nucleus-encoded, plant-specific nucleic-acid-binding protein that localizes predominantly to mitochondria, where it is enriched in mitochondrial nucleoids and associates with mtDNA-containing complexes. It is synthesized with an N-terminal organelle-targeting presequence that is removed upon import, yielding a mature protein whose core is the conserved WHIRLY domain, an ssDNA-binding module typified by the KGKAAL motif; WHIRLY-family structural studies indicate this domain supports oligomerization into tetramers that can further assemble into higher-order particles on long ssDNA. Functionally, WHIRLY2 acts as an architectural nucleoid factor that binds ssDNA largely without strong sequence preference to promote mtDNA organization/compaction and to support mitochondrial genome stability; genetic disruption in Arabidopsis leads to disordered nucleoids and altered mitochondrial ultrastructure (including reduced cristae organization) accompanied by impaired mitochondrial performance, and loss-of-function also compromises mtDNA integrity/repair under stress (e.g., salt stress) with accumulation of aberrant recombination products and heightened stress sensitivity. Collectively, WHIRLY2 links nucleoid structure to mitochondrial morphology and bioenergetic competence, helping maintain mitochondrial genome maintenance and stress-responsive mitochondrial function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT1G71260; ATWHY2; F3I17.9; F3I17_9; WHIRLY 2; WHY2
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