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Invitrogen
WNK1 Recombinant Rabbit Monoclonal Antibody (6C5S6)
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WNK1 Antibody (MA5-35466) in WB
Western blot analysis of WNK1 in mouse kidney. Samples were incubated with WNK1 Monoclonal antibody (Product # MA5-35466) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
产品信息
MA5-35466
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
6C5S6
抗原
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human WNK1 (Q9H4A3)
偶联物
形式
Liquid
浓度
0.53 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MSGGAAEKQS STPGSLFLSP PAPAPKNGSS SDSSVGEKLG AAAADAVTGR TEEYRRRRHT MDKDSRGAAA TTTTTEHRFF RRSVICDSNA TALELPGLPL
靶标信息
WNK1 serine/threonine kinase is a member of the WNK subfamily. WNK1 may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Also, acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, and SGK1. WNK localizes to a segment of kidney involved in salt, potassium, and pH homeostasis, the distal nephron. Mutations in the WNK gene result in Pseudohypaldosteronism and Neuropathy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MGC163339; MGC163341; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; Serine/threonine-protein kinase WNK1; With no K
基因别名: 6430573H23Rik; EG406236; Hsn2; mKIAA0344; Prkwnk1; Wnk1
UniProt ID: (Mouse) P83741
Entrez Gene ID: (Mouse) 232341
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
protein kinase inhibitor activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
chloride channel inhibitor activity
potassium channel inhibitor activity
protein kinase binding
phosphatase binding
protein serine/threonine kinase inhibitor activity
non-receptor serine/threonine protein kinase
positive regulation of systemic arterial blood pressure
protein phosphorylation
ion transport
negative regulation of phosphatase activity
phosphorylation
negative regulation of kinase activity
intracellular signal transduction
protein autophosphorylation
regulation of cellular process
negative regulation of protein serine/threonine kinase activity
negative regulation of pancreatic juice secretion
