Invitrogen

WNT1 Polyclonal Antibody, Biotin, PeproTech®

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WNT1 Polyclonal Antibody, Biotin, PeproTech®

产品信息

500-P250BT-25UG

应用
建议稀释比
已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL
-

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL
-

其他PubMed (Misc)

-
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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived, 38.4 kDa Recombinant Human Wnt-1
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偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2930063

产品详细信息

AA Sequence of recombinant protein: ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Wnt-1. Anti-Human Wnt-1-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hWNT-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human WNT-1 (500-P250) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hWNT-1.

Western Blot: To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hWNT-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P250BT-1MG will be provided as 2 x 500 µg

靶标信息

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Proto-oncogene Int-1 homolog; Proto-oncogene Wnt-1; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)

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基因别名: BMND16; INT1; OI15; WNT1

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UniProt ID: (Human) P04628

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Entrez Gene ID: (Human) 7471

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功能
frizzled binding cytokine activity morphogen activity protein domain specific binding transcription regulatory region DNA binding receptor agonist activity intercellular signal molecule
参与通路
embryonic axis specification branching involved in ureteric bud morphogenesis positive regulation of protein phosphorylation cell-cell signaling myoblast fusion positive regulation of cell proliferation response to wounding positive regulation of lamellipodium assembly negative regulation of cell-substrate adhesion Wnt signaling pathway spinal cord association neuron differentiation diencephalon development central nervous system morphogenesis cerebellum formation forebrain anterior/posterior pattern specification midbrain-hindbrain boundary maturation during brain development negative regulation of cell-cell adhesion negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway ubiquitin-dependent SMAD protein catabolic process midbrain development organ regeneration T cell differentiation in thymus cell proliferation in midbrain inner ear morphogenesis signal transduction in response to DNA damage positive regulation of insulin-like growth factor receptor signaling pathway canonical Wnt signaling pathway involved in negative regulation of apoptotic process cell fate commitment negative regulation of fat cell differentiation positive regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation neuron fate determination neuron development positive regulation of sequence-specific DNA binding transcription factor activity Spemann organizer formation canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway bone development positive regulation of dermatome development hepatocyte differentiation cellular response to peptide hormone stimulus hematopoietic stem cell proliferation positive regulation of canonical Wnt signaling pathway negative regulation of cell aging beta-catenin destruction complex disassembly Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation embryonic brain development negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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