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Bethyl Laboratories
WRN Recombinant Rabbit Monoclonal Antibody (BLR333M)
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WRN Antibody (A700-333) in WB
Detection of human WRN by western blot. Samples: Whole cell lysate (50 µg) from K-562, RKO, LNCaP, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-WRN recombinant monoclonal antibody [BLR333M] (A700-333) used at 1:1,000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).
产品信息
A700-333
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
BLR333M
抗原
Recombinant antibody was purified from cell culture supernatant. Immunogen was a peptide representing a region between residue 1382 and the C-terminus (residue 1432) of human ATP-dependent helicase WRN using the numbering given in entry NP_000544.2 (Gene ID 7486).
偶联物
形式
Liquid
浓度
500 µg/mL
纯化类型
purified
保存液
PBS, pH 6.8-7.4, with 0.1% BSA
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN; DKFZp686C2056; DNA helicase, RecQ-like type 3; exonuclease WRN; RecQ protein-like 2; Werner syndrome protein; Werner syndrome, RecQ helicase-like
基因别名: RECQ3; RECQL2; RECQL3; WRN
UniProt ID: (Human) Q14191
Entrez Gene ID: (Human) 7486
magnesium ion binding
Y-form DNA binding
bubble DNA binding
DNA binding
DNA helicase activity
chromatin binding
ATP-dependent DNA helicase activity
helicase activity
exonuclease activity
protein binding
ATP binding
3'-5' exonuclease activity
four-way junction helicase activity
ATPase activity
manganese ion binding
protein complex binding
protein homodimerization activity
3'-5' DNA helicase activity
ATP-dependent 3'-5' DNA helicase activity
G-quadruplex DNA binding
DNA helicase
telomere maintenance via recombination
telomere maintenance
DNA synthesis involved in DNA repair
strand displacement
replicative cell aging
DNA metabolic process
DNA replication
base-excision repair
double-strand break repair
cellular response to DNA damage stimulus
response to oxidative stress
brain development
aging
cell aging
cellular response to starvation
response to UV-C
multicellular organism aging
protein sumoylation
replication fork processing
nucleolus to nucleoplasm transport
DNA duplex unwinding
regulation of growth rate
regulation of apoptotic process
positive regulation of hydrolase activity
cellular response to gamma radiation
nucleic acid phosphodiester bond hydrolysis
regulation of signal transduction by p53 class mediator
