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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • XBP1 Antibodies

          Rockland

          XBP-1 Polyclonal Antibody

          查看其他29个XBP1抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite XBP-1 Polyclonal Antibody

          • 抗体检测数据 (12)
          XBP-1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          XBP-1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 12

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          XBP-1 Antibody (600-401-H33) in ICC/IF

          Immunofluorescence Validation of XBP-1. Cell: HeLa Cells. Fixation: methanol-fixed. Primary Antibody: Anti-XBP-1 at 10 μg/mL. Secondary: goat anti-rabbit IgG secondary antibody at 1:1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          XBP-1 Antibody in Immunocytochemistry (ICC/IF)
          XBP-1 Antibody in Immunocytochemistry (ICC/IF)
          XBP-1 Antibody in Immunocytochemistry (ICC/IF)
          XBP-1 Antibody in Immunocytochemistry (ICC/IF)
          XBP-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          XBP-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          XBP-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          XBP-1 Antibody in Western Blot (WB)
          XBP-1 Antibody in Western Blot (WB)
          XBP-1 Antibody in Western Blot (WB)
          XBP-1 Antibody in Western Blot (WB)
          XBP-1 Antibody in Western Blot (WB)
          XBP-1 Polyclonal Antibody

          产品信息

          600-401-H33

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          0.25-2 µg/mL
          -

          免疫组化(石蜡) (IHC (P))

          2-5 µg/mL
          -

          免疫细胞化学 (ICC/IF)

          20 µg/mL
          -

          酶联免疫吸附实验 (ELISA)

          Assay-dependent
          -
          产品规格

          种属反应

          Human, Mouse

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          XBP-1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide near the n-terminus of human XBP-1.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.02% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          靶标信息

          XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: OTTHUMP00000028682; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB-5; X-box-binding protein 1; XBP-1

          查看更多 收起

          基因别名: D11Ertd39e; TREB-5; TREB5; XBP-1; XBP1; XBP2

          查看更多 收起

          UniProt ID: (Human) P17861, (Mouse) O35426

          查看更多 收起

          Entrez Gene ID: (Human) 7494, (Mouse) 22433

          查看更多 收起

          功能
          RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding phosphatidylinositol 3-kinase regulatory subunit binding histone deacetylase binding protein heterodimerization activity RNA polymerase II regulatory region sequence-specific DNA binding protease binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding sequence-specific DNA binding transcription regulatory region DNA binding
          参与通路
          angiogenesis liver development endothelial cell proliferation positive regulation of immunoglobulin production transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process fatty acid biosynthetic process autophagy immune response positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response muscle organ development regulation of autophagy negative regulation of myotube differentiation protein transport endoplasmic reticulum unfolded protein response exocrine pancreas development protein destabilization cellular response to nutrient cellular response to insulin stimulus cellular triglyceride homeostasis IRE1-mediated unfolded protein response ATF6-mediated unfolded protein response cellular response to glucose starvation cholesterol homeostasis negative regulation of apoptotic process positive regulation of MHC class II biosynthetic process positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of fat cell differentiation positive regulation of transcription from RNA polymerase II promoter neuron development positive regulation of immunoglobulin secretion fatty acid homeostasis sterol homeostasis adipose tissue development epithelial cell maturation involved in salivary gland development intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to fructose stimulus cellular response to glucose stimulus cellular response to interleukin-4 cellular response to peptide hormone stimulus positive regulation of plasma cell differentiation positive regulation of endoplasmic reticulum unfolded protein response positive regulation of proteasomal protein catabolic process negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of lactation response to insulin-like growth factor stimulus positive regulation of hepatocyte proliferation positive regulation of interleukin-6 secretion negative regulation of transcription from RNA polymerase II promoter positive regulation of protein phosphorylation epithelial cell maturation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter lipid metabolic process transport apoptotic process response to unfolded protein multicellular organism development positive regulation of autophagy phosphatidylinositol 3-kinase signaling cell growth cell differentiation positive regulation of histone methylation regulation of protein stability positive regulation of TOR signaling cellular response to oxidative stress response to endoplasmic reticulum stress cellular response to vascular endothelial growth factor stimulus response to drug glucose homeostasis positive regulation of transcription factor import into nucleus vascular endothelial growth factor receptor signaling pathway response to electrical stimulus serotonin secretion, neurotransmission cellular response to fluid shear stress cellular response to laminar fluid shear stress negative regulation of endoplasmic reticulum unfolded protein response positive regulation of protein acetylation positive regulation of endothelial cell apoptotic process
          It has to be done as per old AB suggested Products section.
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