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Agrisera
XLG2/Extra-large G-protein 2 Polyclonal Antibody
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XLG2/Extra-large G-protein 2 Antibody (AS22-4881) in WB
Samples: Left panel: 50 ng of recombinant At XLG 2 protein Right panel: 20 µg of a total protein from Arabidopsis thaliana wt (wt) and xlg 1,2,3 goa 1-3 quadruple mutant (mut)Samples were separated on 12% SDS-PAGE and blotted for 1 h to PVDF using wet transfer. Blot was blocked with 5% milk for 1 h/RT. Blot was incubated in the primary antibody at a dilution of 1:5,000 for 1 h/4°C with agitation in TBS-T. T... View More
产品信息
AS22-4881
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant XLG2 of Arabidopsis thaliana.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
XLG2 encodes a plant-specific, noncanonical heterotrimeric G protein alpha-like factor that localizes dynamically to the nucleus and cell periphery, consistent with roles in both intracellular signaling and transcriptional control. The protein is structurally distinguished from canonical G alpha subunits by a large N-terminal extension (approximately 400 amino acids) that contains regulatory features including a functional nuclear localization signal, coupled to a C-terminal G alpha-like domain capable of nucleotide binding and hydrolysis. Biochemically, XLG2 binds and hydrolyzes GTP in a Ca2+-dependent manner (rather than the typical Mg2+ dependence of many metazoan G alpha proteins), supporting its classification as an unconventional GTPase. Functionally, XLG2 participates in defense signaling: it is induced during bacterial infection, contributes to pathogen resistance, and can act within an alternative G protein complex via interaction with the Arabidopsis G beta subunit, shaping downstream immune gene activation. In development, XLG2 can interact with the nuclear factor RTV1 and enhance its DNA-binding activity, thereby promoting transcription of floral integrator genes such as FT and SOC1 and influencing flowering time.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT4G34390; extra-large GTP-binding protein 2; F10M10.160; F10M10_160
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