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Invitrogen
YTHDF1 Recombinant Rabbit Monoclonal Antibody (PSH0-23)
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YTHDF1 Antibody (MA5-50793) in IHC (P)
Immunohistochemistry of YTHDF1 in paraffin-embedded mouse hippocampus tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with YTHDF1 recombinant monoclonal antibody (Product # MA5-50793) with a dilution of 1:1,000 for 1 hour a... View More
产品信息
MA5-50793
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH0-23
抗原
Recombinant protein within human YTHDF1 aa 300-559/559.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive control: HepG2 cell lysate, Hela cell lysate, 293T cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HCT116 cell lysate, mouse testis liver tissue lysate, rat testis tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, mouse brain tissue, mouse hippocampus tissue, rat brain tissue, 293, PC-12.
Predicted band size: 61 kDa
Subcellular Location: Cytoplasm, P-body.
靶标信息
Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (PubMed:26046440). Required to facilitate learning and memory formation in the hippocampus by enhancing protein synthesis upon neuronal stimulation: in response to neuronal stimulation, binds to m6A-containing neuronal mRNAs, promoting their translation, thereby contributing to learning and memory. Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts, thereby promoting their translation. Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells. Acts by binding and promoting translation of m6A-containing transcripts encoding proteins involved in lysosomal degradation and phagosome maturation, leading to increased antigen degradation in myeloid dendritic cells. In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DACA-1; DACA-1 homolog; Dermatomyositis associated with cancer putative autoantigen 1; Dermatomyositis associated with cancer putative autoantigen 1 homolog; DF1; YTH domain family 1; YTH domain family protein 1; YTH domain family, member 1; YTH domain-containing family protein 1; YTH N(6)-methyladenosine RNA binding protein 1
基因别名: 2210410K23Rik; 8030473O16; C20orf21; YTHDF1
UniProt ID: (Human) Q9BYJ9, (Mouse) P59326
Entrez Gene ID: (Human) 54915, (Mouse) 228994, (Rat) 296467
