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Invitrogen
ZAK Polyclonal Antibody
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FIGURE: 1 / 2
ZAK Antibody (PA5-79630) in WB
Western blot analysis of MAP3K20 in, Lane 1: human PANC-1 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with ZAK Polyclonal Antibody (Product # PA5-79630) at 0.5 μg/... View More
Product Details
PA5-79630
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human MAP3K20 recombinant protein (Position: F276-E511).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse thymus tissue, human PANC-1 whole cell.
Target Information
ZAK is a leucine zipper-containing mitogen-activated protein related to the MLKs in both sequence and function. Like the MLKs and other MAP3Ks, ZAK activates MAP2Ks. ZAK is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif. This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HCCS-4; Human cervical cancer suppressor gene 4 protein; Leucine zipper- and sterile alpha motif kinase ZAK; Leucine zipper- and sterile alpha motif-containing kinase; Mitogen-activated protein kinase kinase kinase 20; Mitogen-activated protein kinase kinase kinase MLT; Mixed lineage kinase 7; mixed lineage kinase with a leucine zipper and a sterile alpha motif; Mixed lineage kinase-related kinase; mixed lineage kinase-related kinase MRK-beta; MLK-like mitogen-activated protein triple kinase; MLK-related kinase; MRK; sterile alpha motif and leucine zipper containing kinase AZK; Sterile alpha motif- and leucine zipper-containing kinase AZK; sterile-alpha motif and leucine zipper containing kinase AZK
Gene Aliases: AV006891; AZK; B230120H23Rik; HCCS4; MAP3K20; MLK7; mlklak; MLT; MLTK; MLTKalpha; MLTKbeta; MRK; pk; RGD1561394; SFMMP; ZAK
UniProt ID: (Human) Q9NYL2, (Mouse) Q9ESL4
Entrez Gene ID: (Human) 51776, (Mouse) 65964, (Rat) 311743
magnesium ion binding
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
poly(A) RNA binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
immunoglobulin
non-receptor serine/threonine protein kinase
cell cycle checkpoint
DNA damage checkpoint
activation of MAPKK activity
protein phosphorylation
response to stress
cytoskeleton organization
cell cycle arrest
activation of JUN kinase activity
cell death
cell proliferation
response to radiation
cell differentiation
intracellular signal transduction
embryonic digit morphogenesis
positive regulation of apoptotic process
limb development
MAPK cascade
cell cycle
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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