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Invitrogen
ZDHHC20 Polyclonal Antibody
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ZDHHC20 Antibody (PA5-101778) in ICC/IF
Immunocytochemistry analysis of ZDHHC20 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ZDHHC20 polyclonal antibody (Product # PA5-101778) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-101778
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ZDHHC20(Accession Q5W0Z9), corresponding to amino acid residues M71-A121.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total ZDHHC20.
靶标信息
Catalyzes palmitoylation of Cys residues on target proteins (PubMed:27153536, PubMed:29326245). Catalyzes palmitoylation of Cys residues in the cytoplasmic C-terminus of EGFR, and modulates the duration of EGFR signaling by modulating palmitoylation-dependent EGFR internalization and degradation (PubMed:27153536). Has a preference for acyl-CoA with C16 fatty acid chains (PubMed:29326245). Can also utilize acyl-CoA with C14 and C18 fatty acid chains (PubMed:29326245). [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Acyltransferase ZDHHC20; DHHC domain-containing cysteine-rich protein 20; DHHC-20; DHHC-containing protein 20; DHHC20; membrane-associated DHHC24 zinc finger protein; Palmitoyltransferase ZDHHC20; probable palmitoyltransferase ZDHHC20; Zinc finger DHHC domain-containing protein 20; zinc finger, DHHC domain containing 20; zinc finger, DHHC-type containing 20
基因别名: 4933421L13Rik; 5033406L14Rik; AI448102; B230110O18Rik; DHHC-20; HK11; RGD1305755; ZDHHC20
UniProt ID: (Human) Q5W0Z9, (Mouse) Q5Y5T1
Entrez Gene ID: (Human) 253832, (Rat) 305923, (Mouse) 75965
