Invitrogen

ZNF192 Polyclonal Antibody

查看其他8个ZNF192抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

ZNF192 Antibody in Immunocytochemistry (ICC/IF)

ZNF192 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

ZNF192 Antibody (PA5-118368) in ICC/IF

Immunofluorescence staining of ZNF192 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ZNF192 Polyclonal Antibody (Product # PA5-118368, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.

ZNF192 Antibody in Immunocytochemistry (ICC/IF)

ZNF192 Antibody in Western Blot (WB)

ZNF192 Antibody in Immunoprecipitation (IP)

Product Image Gallery

ZNF192 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of ZNF192 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ZNF192 Polyclonal Antibody (Product # PA5-118368, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.

ZNF192 Antibody in Western Blot (WB) — Western Blot using ZNF192 Polyclonal Antibody (Product # PA5-118368) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: U-251MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 65 kDa. Observed band size: 70 kDa.

ZNF192 Antibody in Immunoprecipitation (IP) — ZNF192 Immunoprecipitation using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 µL with ZNF192 Polyclonal Antibody (Product # PA5-118368) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: ZNF192 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 65 kDa. Observed band size: 65 kDa.

产品信息

PA5-118368

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

免疫沉淀 (IP)

1-5 µL/mg of lysate

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human ZNF192/ZKSCAN8 fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7

内含物

0.03% ProClin 300

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

May be involved in transcriptional regulation. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CAML; LD5-1; Zinc finger protein 192; Zinc finger protein with KRAB and SCAN domains 8

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基因别名: LD5-1; ZKSCAN8; ZNF192; ZSCAN40

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UniProt ID: (Human) Q15776

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Entrez Gene ID: (Human) 7745

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transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding metal ion binding C2H2 zinc finger transcription factor

transcription, DNA-templated regulation of transcription, DNA-templated