Invitrogen

ZO-1 Polyclonal Antibody (ZMD.437)

Name: ZO-1 Polyclonal Antibody (ZMD.437)
Brand: Invitrogen
SKU: 40-2300

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82个已发表图片

147 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

ZO-1 Antibody in Immunocytochemistry (ICC/IF)

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ZO-1 Antibody (40-2300) in ICC/IF

Immunofluorescent analysis of ZO-1 (green) in Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 30 minutes at room temperature. Cells were stained with or without ZO-1 rabbit polyclonal antibody (Product # 40-2300), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:1000 for 1 hour at room... View More

ZO-1 Antibody in Immunohistochemistry (IHC)

ZO-1 Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))

ZO-1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

ZO-1 Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

ZO-1 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

ZO-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZO-1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

ZO-1 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

ZO-1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

ZO-1 Antibody in Western Blot, Immunohistochemistry, Immunoprecipitation (WB, IHC, IP)

ZO-1 Antibody in Immunohistochemistry - Free Floating (IHC (Free))

Product Image Gallery

ZO-1 Antibody — Figure 3 TJ protein expression in bEnd3 cells treated with exogenous MMP-9 and GM6001. (A & B) Immunoblot of occludin, claudin-5, ZO-1, and ZO-2 expression levels in Triton X-100-soluble fractions and densitometric analyses of bEnd3 cells incubated with culture medium alone, with 100 ng/mL of MMP-9, and with MMP-9 and 100 nM of GM6001. (C & D) Immunoblot of TJ proteins in Triton X-100-insoluble fractions and densitometric analyses of bEnd3 cells in the three groups. GAPDH expression was used as loading control. Values are expressed as mean percentage +- SD (* p <0.05, ** p <0.01). {TM}

Immunogen image

Immunogen Image ZO-1 Polyclonal Antibody (ZMD.437)

产品信息

40-2300

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1-2 µg/mL

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免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫组化（冰冻） (IHC (F))

2 µg/mL

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免疫组织化学（悬浮细胞） (IHC (Free))

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免疫细胞化学 (ICC/IF)

5-10 µg/mL

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免疫沉淀 (IP)

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亲和纯化 (AP)

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其他PubMed (Misc)

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产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

C. elegans, Chicken, Dog, Human, Mouse, Pig, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

克隆号

ZMD.437

抗原

Synthetic peptide derived from the N-terminal region of human, dog, mouse, and rat ZO-1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533457

产品详细信息

This antibody reacts with the ZO-1 protein. Based on sequence homology, reactivity with ZO-2 or ZO-3 is not expected. 40-2300 has been successfully used in immunofluorescence and immunohistochemistry. However, for western blotting we recommend the use of monoclonal anti-ZO-1 antibody (Cat. No. 339100), or other polyclonal ZO-1 antibodies (Cat. Nos. 617300 or 402200).

Reactivity has been confirmed with MDCK-II, Caco-2, Rat-1, and NRK52E cells by immunofluorescence. Reactivity has also been confirmed with frozen mouse liver, blood vessel (in heart), and mossy fiber terminal (in brain) tissues by immunohistochemistry and immunofluorescence, and with formalin-fixed, paraffin-embedded human pancreas, prostate, and small intestine tissues by immunohistochemistry. This antibody (or Cat. No. 187430) is recommended for immunohistochemical detection of ZO-1.

For immunohistochemistry in formalin-fixed, paraffin-embedded tissues, tissue pretreatment with pepsin is required prior to staining. Some lots of this antibody have successfully been used in western blotting using human, dog and rat cell lysates. For immunofluorescence methanol fixation is preferred even though paraformaldehyde fixation will also work.

靶标信息

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

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基因别名: TJP1; ZO-1; ZO1

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UniProt ID: (Dog) O97758, (Human) Q07157, (Mouse) P39447

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Entrez Gene ID: (Dog) 403752, (Human) 7082, (Mouse) 21872, (Rat) 292994

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功能

protein binding calmodulin binding protein domain specific binding cadherin binding involved in cell-cell adhesion protein C-terminus binding tight junction

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