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Invitrogen
ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 488
高级验证
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
图: 1 / 4
ZO-1 Antibody (MA3-39100-A488) in ICC/IF
Knockout of ZO-1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID # CRISPR533979_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on Caco-2 wild type cells (panel a,d), Caco-2 Cas9 cells (panel b,e) and Caco-2 ZO-1KO cells (panel c, f). Cells were fixed, permeabilized, and labeled with ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 488 (Product # MA3-39100-A488, 1:500 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antif... View More
产品信息
MA3-39100-A488
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
ZO1-1A12
抗原
Human recombinant ZO-1 fusion protein encompassing amino acids 334-634
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2663169
靶标信息
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
基因别名: TJP1; ZO-1; ZO1
UniProt ID: (Dog) O97758, (Human) Q07157
Entrez Gene ID: (Dog) 403752, (Human) 7082
