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ZO-1 Antibody (740002MP647) in ICC/IF
Immunofluorescent analysis of ZO-1 was performed using 70% confluent Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with ZO-1 recombinant mouse monoclonal antibody (ZO1-1A12), Alexa Fluor™ Plus 647 Antibody (740002MP647, 1:2,000 dilution) at 4 degree Celsius overnight. Panel a) shows representative images of cells that were stained for detection and localization of ZO-1 (red). Panel... View More
产品信息
740002MP647
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
ZO1-1A12
抗原
Human recombinant ZO-1 fusion protein encompassing amino acids 334-634
偶联物
激发/发射光谱
658/675 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.5
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
AB_3093163
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This ZO-1 antibody has been tested by immunohistochemistry of mouse testis tissue and immunocytochemistry and flow cytometric analysis of Caco-2 cells. This may be used at 0.063-0.5 µg per test for flow cytometry. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
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生物信息学
蛋白别名: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
基因别名: TJP1; ZO-1; ZO1
UniProt ID: (Human) Q07157
Entrez Gene ID: (Human) 7082
