Invitrogen

ZSCAN2 Polyclonal Antibody

查看其他3个ZSCAN2抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

ZSCAN2 Antibody in Immunocytochemistry (ICC/IF)

ZSCAN2 Antibody in Immunocytochemistry (ICC/IF)

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ZSCAN2 Antibody (PA5-100769) in ICC/IF

Immunocytochemistry analysis of ZSCAN2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ZSCAN2 polyclonal antibody (Product # PA5-100769) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

ZSCAN2 Antibody in Immunocytochemistry (ICC/IF)

ZSCAN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZSCAN2 Antibody in Western Blot (WB)

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ZSCAN2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of ZSCAN2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ZSCAN2 polyclonal antibody (Product # PA5-100769) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

ZSCAN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ZSCAN2 in rat brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with ZSCAN2 polyclonal antibody (Product # PA5-100769) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

ZSCAN2 Antibody in Western Blot (WB) — Western blot analysis of ZSCAN2 in HEK293 cells lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with ZSCAN2 polyclonal antibody (Product # PA5-100769).

Immunogen image

ZSCAN2 Polyclonal Antibody

Immunogen Image ZSCAN2 Polyclonal Antibody

产品信息

PA5-100769

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human ZSCAN2(Accession Q7Z7L9), corresponding to amino acid residues I322-S372.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total ZSCAN2.

靶标信息

May be involved in transcriptional regulation during the post-meiotic stages of spermatogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Zfp-29; Zinc finger and SCAN domain-containing protein 2; Zinc finger protein 29; Zinc finger protein 29 homolog; Zinc finger protein 854

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基因别名: Zfp-29; ZFP29; ZNF854; ZSCAN2

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UniProt ID: (Human) Q7Z7L9, (Mouse) Q07230

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Entrez Gene ID: (Human) 54993, (Mouse) 22691

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RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding metal ion binding nucleic acid binding DNA binding DNA-binding transcription factor C2H2 zinc finger transcription factor gene-specific transcriptional regulator

transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development spermatogenesis cell differentiation