Search
Agrisera
aadA1/Aminoglycoside adenyltransferase (chloroplast transformation marker) Polyclonal Antibody, AP
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
产品信息
AS09-580-ALP
产品规格
种属反应
Algae,
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from known aadA1 protein sequences.
偶联物
AP
AP
AP
形式
Liquid
规格
40 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Specific Species Reactivity: aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
靶标信息
Aminoglycoside adenyltransferase (AadA; aminoglycoside O-nucleotidyltransferase) is a bacterial cytosolic enzyme typically encoded by horizontally mobile antibiotic-resistance determinants (commonly plasmid- and integron-associated) and produced as a soluble monomeric protein. Structurally, AadA adopts a two-domain architecture comprising an N-terminal nucleotidyltransferase (NTase) catalytic domain and a C-terminal alpha-helical bundle domain; the active site lies in an interdomain cleft and undergoes ligand-coupled conformational rearrangements, with ATP binding occurring prior to aminoglycoside binding to properly position the domains for catalysis. Functionally, AadA mediates aminoglycoside resistance by catalyzing a Mg2+-dependent transfer of AMP from ATP onto hydroxyl groups of target antibiotics, including O-adenylation at the 3''-hydroxyl of streptomycin and the 9-hydroxyl of spectinomycin, thereby reducing drug affinity for the ribosome and diminishing inhibition of protein synthesis; mutational and structural studies support a key role for a conserved glutamate (e.g., Glu87 in Salmonella enterica AadA) as the catalytic base and identify basic residues important for ATP coordination during the adenylation reaction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: aadA; aadA1; aadA10; aadA14; aadA2; aadA21; aadA23; aadA4; aadA5; aadA6; aadA7; aadA8; aadA9
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
