Invitrogen

alpha Adaptin Monoclonal Antibody (AC1-M11)

Name: alpha Adaptin Monoclonal Antibody (AC1-M11)
Brand: Invitrogen
SKU: MA3-061

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6个已发表图片

26 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

alpha Adaptin Antibody in Immunocytochemistry (ICC/IF)

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alpha Adaptin Antibody (MA3-061) in ICC/IF

Immunofluorescent analysis of alpha Adaptin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Adaptin (AC1-M11) Mouse Monoclonal Antibody (Product # MA3-061) at 2 µg/mL in 0.1% BSA and incubated ... View More

alpha Adaptin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Adaptin Antibody in Western Blot (WB)

alpha Adaptin Antibody in Immunoprecipitation (IP)

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产品信息

MA3-061

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

1:100

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免疫沉淀 (IP)

Assay-dependent

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亲和纯化 (AP)

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产品规格

种属反应

Amphibian, Bovine, Chicken, Human, Mouse, Non-human primate, Pig, Rat

已发表种属

Amphibian, Bovine, C. elegans, Human, Mouse, Non-human primate, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

AC1-M11

抗原

Purified bovine brain adaptor complexes.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2056321

产品详细信息

MA3-061 detects alpha-adaptin from bovine, chicken, frog, human, monkey, mouse, pig and rat. This antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons.

MA3-061 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody recognizes an ~100 kDa doublet representing the two isoforms of alpha-adaptin from pig brain extract. Immunofluorescence staining of alpha-adaptin in transfected COS cells with MA3-061A yields distinct punctate dots on the plasma membrane.

The MA3-061 antigen is purified bovine brain adaptor complexes.

靶标信息

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; Adaptor protein complex AP-2 subunit alpha-1; adaptor protein complex AP-2, alpha 1 subunit; adaptor related protein complex 2 alpha 1 subunit; adaptor related protein complex 2, alpha 1 subunit; Adaptor-related protein complex 2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; adaptor-related protein complex AP-2, alpha 1 subunit; ADTAB; Alpha-adaptin A; alpha-adaptin C; Alpha1-adaptin; alpha2-adaptin; AP-2 complex subunit alpha-1; AP2; CLAPA2; Clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; HIP-9; HIP9; HYPJ; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

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基因别名: ADTAA; AP2-ALPHA; AP2A1; CLAPA1

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UniProt ID: (Human) O95782, (Mouse) P17426