Invitrogen

alpha/beta Tubulin Monoclonal Antibody (TU-10)

Name: alpha\/beta Tubulin Monoclonal Antibody (TU-10)
Brand: Invitrogen
SKU: MA1-19400

1个已发表图片

2 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)
已发表图片 (1)

alpha/beta Tubulin Antibody in Immunocytochemistry (ICC/IF)

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alpha/beta Tubulin Antibody (MA1-19400) in ICC/IF

Immunofluorescence analysis of alpha/beta Tubulin was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha/beta Tubulin (TU-10) Mouse Monoclonal Antibody (Product # MA1-19400) at 2µg/mL in 0.1% BSA and in... View More

alpha/beta Tubulin Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image alpha/beta Tubulin Monoclonal Antibody (TU-10)

产品信息

MA1-19400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫细胞化学 (ICC/IF)

Assay-dependent

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流式细胞分析 (Flow)

8-12 µg/mL

亲和纯化 (AP)

- -

产品规格

种属反应

Human, Many, Mouse, Pig, Rat

已发表种属

Human

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

TU-10

抗原

Microtubule proteins from porcine brain.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

TBS, pH 8

内含物

15mM sodium azide

保存条件

4°C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_1070282

产品详细信息

This antibody recognizes alpha, beta-tubulin heterodimer, a basic intracellular structural unit of microtubules. Alpha- and beta-tubulins form approximately 100 kDa tubulin heterodimer, a globular protein that polymerizes to form microtubules.

Western Blot: Reducing conditions.

靶标信息

Tubulin protein is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution. Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Anti-microtubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Alpha-tubulin 1; Alpha-tubulin 3; Alpha-tubulin isotype M-alpha-1; beta 5-tubulin; beta Ib tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Tubulin alpha; tubulin alpha 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-3 chain; Tubulin B-alpha-1; tubulin beta; Tubulin beta chain; Tubulin beta-1 chain; Tubulin beta-5 chain; tubulin, alpha 1; tubulin, alpha 1a; tubulin, alpha, brain-specific; tubulin, beta 1 class VI; tubulin, beta 2A class IIa; tubulin, beta class I; tubulin, beta family 1; tubulin, beta polypeptide; α/β-tubulin

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基因别名: 2810484G07Rik; B-ALPHA-1; CDCBM6; CSCSC1; LIS3; M(beta)1; M40; OK/SW-cl.56; Tuba-1; Tuba1; TUBA1A; TUBA3; TUBB; TUBB1; TUBB2A; TUBB5