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beta-2 Defensin Antibody (500-P161GBT-1MG) in WB
Western Blot: To detect hBD-2 by Western Blot analysis beta-2 Defensin Polyclonal Antibody, Biotin (Product # 500-P161GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P161GBT-1MG
产品规格
已发表种属
Human
宿主/亚型
Goat
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human BD-2
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929559
产品详细信息
AA Sequence of recombinant protein: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human BD-2. Anti-Human BD-2-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBD-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-2 (500-P161G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-2.
Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P161GBT-1MG will be provided as 2 x 500 µg
靶标信息
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
