Invitrogen

beta-3 Tubulin Monoclonal Antibody (2G10-TB3), Alexa Fluor™ 488, eBioscience™

Name: beta-3 Tubulin Monoclonal Antibody (2G10-TB3), Alexa Fluor\u2122 488, eBioscience\u2122
Brand: Invitrogen
SKU: 53-4510-82

高级验证

4个已发表图片

8 篇参考文献

查看其他38个Beta III Tubulin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)

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beta-3 Tubulin Antibody (53-4510-82) in ICC/IF

Knockout of beta-3 Tubulin (TUBB3) was achieved by CRISPR-Cas9 genome editing. Immunofluorescence analysis was performed on wild type U-87 MG cells (panel a,d), U-87 MG Cas9 cells (panels b,e) and U-87 MG beta-3 Tubulin KO cells (panel c,f). Cells were fixed, permeabilized, and labelled with beta-3 Tubulin Monoclonal Antibody, Alexa Fluor® 488 (Product # 53-4510-82) (10 µg/mL). Nuclei (blue) were stained us... View More

beta-3 Tubulin Antibody in Flow Cytometry (Flow)

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产品信息

53-4510-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-Dependent

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免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

10 µg/mL

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流式细胞分析 (Flow)

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产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

C. elegans, Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

2G10-TB3

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574421

产品详细信息

Description: The 2G10 monoclonal antibody recognizes beta tubulin class III, a member of the large family of tubulins that are components of the microtubules of the cytoskeletal network. Beta tubulin class III expression is exclusively found in neuronal cells of adult mammals. Although expression has been mainly found in neuronal cells, during fetal development, transient expression has been documented in both glial and neuronal precursors localized to the subventricular zone. Identification of beta tubulin class III expression is useful as a structural marker for developing and mature neural cells. Given the high degree of conservation of this protein across species, the 2G10 clone is expected to recognize human, mouse, rat, and bovine beta tubulin class III.

Applications Reported: This 2G10-TB3 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This 2G10-TB3 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4

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基因别名: 3200002H15Rik; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; TUBB3; TUBB4