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Invitrogen
beta Actin Polyclonal Antibody
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FIGURE: 1 / 2
beta Actin Antibody (PA5-72633) in WB
Western blot analysis of beta Actin in various lysates. Samples were incubated with beta Actin polyclonal antibody (Product # PA5-72633) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Zebrafish lysate; Lane 2: ZF4 whole cell lysate. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-72633
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Zebrafish
Published species
C. elegans,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 188-215 amino acids from the Central region of DANRE actba
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Actin, cytoplasmic 1; B-ACTZF; beta-actin; Beta-actin-1
Gene Aliases: ACTB; actba; B-ACTZF; bact; bactin1; bactzf; wu:fd18f05
UniProt ID: (Zebrafish) Q7ZVI7
Entrez Gene ID: (Zebrafish) 57934
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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