Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
beta Actin Monoclonal Antibody (AC-15)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 5
beta Actin Antibody (AM4302) in ICC/IF
An siRNA targeting beta-actin was labeled with Cy™3 using the Silencer™ siRNA Labeling Kit. The labeled siRNA was transfected into HeLa cells and cells were analyzed 96 hours later using a beta Actin monoclonal antibody (Product # AM4302). Green: beta-actin protein detected with anti-ß-actin (Ambion) and a FITC labeled secondary antibody. Red: Cy3 labeled siRNA. Blue: DAPI stained nuclei. (Cy™3 is a tradema... View More
产品信息
AM4302
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Chicken,
Fruit fly,
Cat,
Guinea pig,
Human,
Sheep,
Pig,
Rabbit
已发表种属
Bacteria,
Bovine,
C. elegans,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Sheep,
Virus,
Zebrafish
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
AC-15
抗原
A slightly modified synthetic beta-cytoplasmic actin N-terminal peptide,Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
15mM sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
运输条件
Dry ice
RRID
产品详细信息
The anti-beta-actin monoclonal antibody can be used to analyze beta-actin protein expression in experimental cells by immunofluorescence and western blot and has been specifically used in combination with beta-actin siRNA control to study the specificity and effectiveness of the RNA Interference (RNAi) effect at the protein level.
The antibody reacts with beta-actin expressing cells in human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, leech and fruit fly tissues, but not in amoeba.
Use Anti-beta-actin for detection of beta-actin by ELISA, immunohistology, western blotting, and immunofluorescence. The antibody specifically recognizes beta-actin in a wide variety of tissues and species using immunoblotting, immunofluorescent staining of cultured cell lines, and immunohistochemistry. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Ethanol, B5, methacarn, or Bouin's solutions may also be used as fixatives.
靶标信息
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: actin, beta; actin, beta, cytoplasmic; Actin, cytoplasmic 1; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoskeletal beta actin; cytoskeletal protein; gamma non-muscle actin; PS1TP5-binding protein 1
基因别名: ACT-5; ACTB; actin; Bact; BRWS1; PS1TP5BP1
UniProt ID: (Sheep) P60713, (Rabbit) P29751, (Bovine) P60712, (Human) P60709, (Pig) Q6QAQ1, (Chicken) P60706, (Dog) O18840, (Guinea pig) Q71FK5
Entrez Gene ID: (Sheep) 443052, (Rabbit) 100009272, (Bovine) 280979, (Human) 60, (Pig) 414396, (Chicken) 396526, (Dog) 403580, (Guinea pig) 100135470
protein binding
ATP binding
kinesin binding
Tat protein binding
identical protein binding
nitric-oxide synthase binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
structural constituent of cytoskeleton
nucleosomal DNA binding
protein kinase binding
structural constituent of postsynaptic actin cytoskeleton
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
morphogenesis of a polarized epithelium
establishment or maintenance of cell polarity
regulation of transmembrane transporter activity
negative regulation of protein binding
adherens junction assembly
apical protein localization
synaptic vesicle endocytosis
regulation of norepinephrine uptake
protein localization to adherens junction
cellular response to cytochalasin B
postsynaptic actin cytoskeleton organization
regulation of protein localization to plasma membrane
retina homeostasis
movement of cell or subcellular component
cytoskeleton organization
substantia nigra development
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
axonogenesis
cell motility
