Invitrogen

beta Arrestin 1 Monoclonal Antibody (ZA005)

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问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

beta Arrestin 1 Antibody in Immunocytochemistry (ICC/IF)

beta Arrestin 1 Antibody in Immunocytochemistry (ICC/IF)

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beta Arrestin 1 Antibody (39-5000) in ICC/IF

Immunofluorescence analysis of beta-Arrestin 1 was done on 70% confluent log phase T47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta-Arrestin 1 (ZA005) Mouse Monoclonal Antibody (Product # 39-5000) at 2 µg/mL in 0.1% BSA and incubated for 3 ho... View More

beta Arrestin 1 Antibody in Immunocytochemistry (ICC/IF)

beta Arrestin 1 Antibody in Western Blot (WB)

beta Arrestin 1 Antibody in Western Blot (WB)

Product Image Gallery

beta Arrestin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of beta-Arrestin 1 was done on 70% confluent log phase T47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta-Arrestin 1 (ZA005) Mouse Monoclonal Antibody (Product # 39-5000) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

beta Arrestin 1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of T-47D (Lane 1), Hep G2 (Lane 2), tissue extracts (30 µg lysate) of Mouse Brain (Lane 3) and Rat Brain (Lane 4). The blot was probed with Anti-beta Arrestin 1 Monoclonal Antibody (Product # 39-5000, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 47 kDa band corresponding to beta Arrestin 1 was observed across the cell lines and tissue extracts tested.

beta Arrestin 1 Antibody in Western Blot (WB) — Western blot analysis of PC-12 cell lysates using Zymed Ms anti-beta-Arrestin 1 (Product # 39-5000).

Immunogen image

beta Arrestin 1 Monoclonal Antibody (ZA005)

Immunogen Image beta Arrestin 1 Monoclonal Antibody (ZA005)

产品信息

39-5000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

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免疫细胞化学 (ICC/IF)

2 µg/mL

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

免疫沉淀 (IP)

-

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其他PubMed (Misc)

-

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ZA005

抗原

Synthetic peptide derived from the C-terminal region of human beta-arrestin 1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: arrestin; arrestin 2; Arrestin beta-1; arrestin, beta 1; beta-arrestin; Beta-arrestin-1; beta-arrestin1; Non-visual arrestin-2

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基因别名: 1200006I17Rik; ARB1; ARR1; ARRB1; AW208571; BARRES; G430100A01Rik

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UniProt ID: (Human) P49407, (Rat) P29066, (Mouse) Q8BWG8

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Entrez Gene ID: (Human) 408, (Rat) 25387, (Mouse) 109689

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histone acetyltransferase activity enzyme inhibitor activity GTPase activator activity insulin-like growth factor receptor binding protein binding transcription factor binding estrogen receptor binding mitogen-activated protein kinase kinase binding ubiquitin protein ligase binding alpha-1A adrenergic receptor binding alpha-1B adrenergic receptor binding angiotensin receptor binding follicle-stimulating hormone receptor binding V2 vasopressin receptor binding AP-2 adaptor complex binding clathrin adaptor activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process transcription regulatory region DNA binding ion channel binding protein phosphorylated amino acid binding G-protein coupled receptor binding clathrin binding phosphoprotein binding scaffold/adaptor protein

activation of MAPK activity positive regulation of protein phosphorylation G-protein coupled receptor internalization positive regulation of receptor internalization transcription from RNA polymerase II promoter apoptotic process phototransduction protein transport protein ubiquitination platelet activation negative regulation of protein ubiquitination positive regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity positive regulation of protein binding negative regulation of interleukin-6 production negative regulation of interleukin-8 production positive regulation of peptidyl-serine phosphorylation negative regulation of GTPase activity positive regulation of smooth muscle cell apoptotic process positive regulation of Rho protein signal transduction positive regulation of histone acetylation positive regulation of insulin secretion involved in cellular response to glucose stimulus response to antidepressant follicle-stimulating hormone signaling pathway stress fiber assembly negative regulation of cysteine-type endopeptidase activity involved in apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of GTPase activity positive regulation of transcription from RNA polymerase II promoter negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of histone H4 acetylation negative regulation of protein phosphorylation desensitization of G-protein coupled receptor protein signaling pathway by arrestin endocytosis receptor-mediated endocytosis G-protein coupled receptor signaling pathway adenylate cyclase-modulating G-protein coupled receptor signaling pathway regulation of G-protein coupled receptor protein signaling pathway sensory perception of pain regulation of cAMP biosynthetic process regulation of cAMP catabolic process sensory perception of touch transcription, DNA-templated regulation of transcription, DNA-templated transport signal transduction negative regulation of signal transduction