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Invitrogen
beta COP Polyclonal Antibody
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beta COP Antibody (PA1-061) in ICC/IF
Immunofluorescent analysis of beta COP was performed using 70% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta COP Rabbit Polyclonal Antibody (Product # PA1-061) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
产品信息
PA1-061
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Bovine,
C. elegans,
Hamster,
Human,
Mouse,
Non-human primate,
Rat,
Yeast
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-061 detects Golgi beta-coatomer protein (beta-COP) from mouse, human and rat tissues.
PA1-061 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110kDa protein which represents beta-COP from rat brain homogenate. Indirect immunofluorescence staining of beta-COP in NIH-3T3 cells with PA1-061 results in beta-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.
The PA1-061 immunogen is a synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP. This sequence is completely conserved in human beta-COP.
靶标信息
This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: beta coat protein; Beta-coat protein; Beta-COP; coatomer protein complex, subunit beta 1; Coatomer subunit beta; DKFZp761K102; FLJ10341; FLJ46444; FLJ57957; receptor for activated C-kinase-2
基因别名: 2610019B04Rik; COPB; COPB1; MSTP026; Rack2
UniProt ID: (Human) P53618, (Mouse) Q9JIF7, (Rat) P23514
Entrez Gene ID: (Human) 1315, (Mouse) 70349, (Rat) 114023
