Rockland

beta TrCP2 Polyclonal Antibody

Name: beta TrCP2 Polyclonal Antibody
Brand: Rockland
SKU: 600-401-483

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

beta TrCP2 Antibody in Western Blot (WB)

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beta TrCP2 Antibody (600-401-483) in WB

Western blot using Rocklands affinity purified anti-bTrCP2 antibody shows detection of mouse and human bTrCP2 (arrowhead) in NIH3T3 (lane 1) and 293 (lane 2) whole cell lysates, respectively. The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted. The identity of faint higher molecular weight bands is not known. The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4°C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG (p/n 611-103-122) diluted 1:20,000 in blocking buffer (p/n... View More

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Immunogen image

Immunogen Image beta TrCP2 Polyclonal Antibody

产品信息

600-401-483

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500- 1:2,000

免疫细胞化学 (ICC/IF)

Assay-dependent

酶联免疫吸附实验 (ELISA)

1:10,000-1:100,000

免疫沉淀 (IP)

1:200-1:1,000

产品规格

种属反应

Bovine, Dog, Chicken, Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human βTrCP2 protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.8 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody reacts with human betaTrCP2 protein. A BLAST analysis was used to suggest cross-reactivity with betaTrCP2 from human, mouse, dog, bovine, chimpanzee, chicken and horse based on a 100% homology with the immunizing sequence. Cross-reactivity with betaTrCP2 from other sources has not been determined.

靶标信息

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: beta-transducin repeat-containing protein 2; F-box and WD repeats protein beta-TrCP2; F-box and WD-40 domain protein 11; F-box and WD-40 domain protein 1B; F-box protein Fbw1b; F-box/WD repeat-containing protein 11; F-box/WD repeat-containing protein 1B; Homologous to Slimb protein; HOS

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基因别名: 2310065A07Rik; AA536858; BTRC2; BTRCP2; Fbw11; FBW1B; FBXW11; FBXW1B; HOS; KIAA0696

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UniProt ID: (Human) Q9UKB1, (Mouse) Q5SRY7

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Entrez Gene ID: (Chicken) 416181, (Dog) 479291, (Human) 23291, (Mouse) 103583

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功能

ubiquitin-protein transferase activity protein binding protein dimerization activity ubiquitin protein ligase activity

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