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Invitrogen
beta Tubulin Polyclonal Antibody
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FIGURE: 1 / 22
beta Tubulin Antibody (PA1-16947) in ICC/IF
Immunofluorescence analysis of beta Tubulin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta Tubulin Rabbit Polyclonal Antibody (Product # PA1-16947) at 2 µg/mL in 0.1% BSA and incubated for 3 ho... View More
Product Details
PA1-16947
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
C. elegans,
Chicken,
Hamster,
Human,
Insect,
Invertebrate,
Mouse,
Non-human primate,
Pig,
Rat,
Xenopus,
Zebrafish
Published species
C. elegans,
Human,
Mouse,
Rhesus monkey
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: antigen standard for TUBB (transient overexpression lysate), Hela whole cell extract.
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta 5-tubulin; beta Ib tubulin; beta tubulin; beta tubulin 1; beta-4 tubulin; beta-4-tubulin; beta-5 tubulin; beta-tubulin; Beta-tubulin class-I; Beta-tubulin class-II; beta-tubulin class-III; Beta-tubulin class-V; Beta-tubulin class-VI; Beta-tubulin isotype I; beta-tubulin T beta15 (aa 1-445); beta5-tubulin; betaIII-tubulin; c(beta)7 tubulin; class I beta tubulin; Class I beta-tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class V beta tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; T beta-15; TUBB; tubb beta-5; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 4'; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; tubulin beta-1; Tubulin beta-1 chain; Tubulin beta-2 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-7 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta 3; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 5; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta family 1; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 3; tubulin, beta, 4; tubulin, beta, 5; unnamed protein product; zbetaI
Gene Aliases: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA506K6.1; bA631M21.2; BB220206; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; fb40f09; FEOM3; HsT1601; I79_000194; I79_012097; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; PMGYSA; RGD1305887; RGD1309427; si:ch211-122i22.4; si:ch211-9a18.2; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB2B; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8; wu:fb40f09; XELAEV_18039222mg; XLOT; zgc:112515; zgc:123194
UniProt ID: (Chicken) P09244, (Human) P07437, (Pig) Q767L7, (Bovine) Q2KJD0, (Chicken) P09207, (Human) Q9H4B7, (Human) Q13885, (Human) A8K068, (Chicken) P32882, (Bovine) Q6B856, (Bovine) Q2T9S0, (Human) Q13509, (Bovine) Q3ZBU7, (Human) P04350, (Human) Q9BUF5, (Chicken) P09653, (Bovine) Q2HJ81, (Human) Q3ZCM7, (Mouse) A2AQ07, (Rat) P85108, (Mouse) Q7TMM9, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Mouse) Q9ERD7, (Rat) Q4QRB4, (Mouse) Q9D6F9, (Mouse) P99024, (Chinese hamster) P69893, (Rat) P69897, (Mouse) Q922F4, (Xenopus) Q91575
Entrez Gene ID: (Chinese hamster) 100761889, (Chicken) 396254, (Human) 203068, (Pig) 733686, (Bovine) 615087, (Chicken) 396427, (Pig) 100134983, (Bovine) 541271, (Human) 81027, (Chicken) 768337, (Human) 7280, (Human) 347733, (Chicken) 420883, (Bovine) 281555, (Pig) 100153507, (Bovine) 768070, (Human) 10381, (Chicken) 431043, (Bovine) 540236, (Human) 10382, (Pig) 100736584, (Pig) 100623583, (Human) 84617, (Chicken) 421037, (Bovine) 534206, (Human) 347688, (Chinese hamster) 100689088, (Mouse) 545486, (Chinese hamster) 100772797, (Rat) 679312, (Rat) 498736, (Mouse) 22151, (Mouse) 73710, (Rat) 291081, (Mouse) 22152, (Rat) 246118, (Chinese hamster) 100760055, (Chinese hamster) 100763390, (Rat) 29213, (Mouse) 22153, (Mouse) 22154, (Chinese hamster) 100689091, (Rat) 29214, (Chinese hamster) 100689363, (Mouse) 67951, (Rat) 307351, (Xenopus) 380418, (Zebrafish) 558552, (Zebrafish) 641421, (Zebrafish) 386701, (Zebrafish) 550458
GTPase activity
structural constituent of cytoskeleton
GTP binding
ubiquitin protein ligase binding
MHC class I protein binding
structural molecule activity
protein binding
protein domain specific binding
protein complex binding
GTPase activating protein binding
calcium ion binding
protein heterodimerization activity
netrin receptor binding
peptide binding
molecular_function
nucleotide binding
tubulin
microtubule cytoskeleton organization
mitotic cell cycle
microtubule-based process
regulation of synapse organization
spindle assembly
G2/M transition of mitotic cell cycle
movement of cell or subcellular component
cellular process
cytoskeleton-dependent intracellular transport
natural killer cell mediated cytotoxicity
cell division
cytoskeleton organization
neuron migration
negative regulation of microtubule polymerization
positive regulation of axon guidance
axon guidance
netrin-activated signaling pathway
dorsal root ganglion development
mitotic nuclear division
neuron differentiation
biological_process
protein polymerization
metabolic process
cell projection organization
fin regeneration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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