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Invitrogen
c-Jun Recombinant Rabbit Monoclonal Antibody (c-Jun-Clone 132)
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c-Jun Antibody (704993) in WB
Western blot was performed using c-Jun Recombinant Rabbit Monoclonal Antibody (c-Jun-Clone 132) (Product # 704993) and ~40 kDa band corresponding to c-Jun was observed across cell lines tested. Modified whole cell extracts (1% SDS) (20 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), U-87 MG (Lane 3), U-2 OS (Lane 4) and BT-549 (Lane 5) were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB33001) by iBlot™3 Western Blot ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
704993
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
c-Jun-Clone 132
抗原
Protein corresponding to human c-Jun [aa231-aa310]
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
产品详细信息
Recombinant Rabbit monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activator protein 1; AP1; cjun; JUND; p39; Proto-oncogene c-Jun; Transcription factor AP-1 subunit Jun; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog
基因别名: JUN
Entrez Gene ID: (Human) 3725
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
