Invitrogen

c-MAF Recombinant Mouse Monoclonal Antibody (sym0F1), Alexa Fluor™ Plus 647

Name: c-MAF Recombinant Mouse Monoclonal Antibody (sym0F1), Alexa Fluor\u2122 Plus 647
Brand: Invitrogen
SKU: 740099MP647

高级验证

查看其他26个c-MAF抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)
高级验证 (1)

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c-MAF Antibody (740099MP647) in Flow

Flow cytometry analysis of c-MAF Recombinant Mouse Monoclonal Antibody (sym0F1), Alexa Fluor™ Plus 647 (Product # 740099MP647) was performed using RPMI 8226 and Daudi cells. Cells were fixed with eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00), permeabilized and stained with 0.5 µg of the c-MAF Recombinant Mouse Monoclonal Antibody (sym0F1), Alexa Fluor™ Plus 647 (Product # 740099MP647). Higher expression of c-MAF was observed in RPMI 8226 (pink histogram) in comparison to Daudi (blue histogram). Viable c... View More

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c-MAF Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. c-MAF Recombinant Mouse Monoclonal Antibody (sym0F1), Alexa Fluor™ Plus 647 (Product # 740099MP647) specifically detects c-MAF expression in RPMI 8226 (pink histogram) while no expression of c-MAF is seen in Daudi cells (blue histogram). {RE}

产品信息

740099MP647

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

sym0F1

偶联物

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

激发/发射光谱

658/675 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

proprietary buffer, pH 6.3-7.3

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors. The leucine-zipper domain is involved in the interaction with LRPICD. There are two forms of human c-maf mRNA, c-maf-long and c-maf-short. It is identified in the genome of the acute transforming avian retrovirus AS42. c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE). It regulates Th2 differentiation and lineage-specific hematopoiesis. c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages. Chromosomal aberration involving maf is found in some forms of multiple myeloma. It is expressed in myeloma cell lines and resting monocytes/macrophages.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; c-maf proto-oncogene; MGC71685; Proto-oncogene c-Maf; T lymphocyte c-maf long form; Transcription factor Maf; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog

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基因别名: AYGRP; c-MAF; CCA4; CTRCT21; MAF

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UniProt ID: (Human) O75444

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Entrez Gene ID: (Human) 4094

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功能

transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein binding sequence-specific DNA binding basic leucine zipper transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter cytokine production regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter regulation of chondrocyte differentiation positive regulation of transcription from RNA polymerase II promoter cell development inner ear development lens fiber cell differentiation