Invitrogen

c-Met Monoclonal Antibody (3D4)

Name: c-Met Monoclonal Antibody (3D4)
Brand: Invitrogen
SKU: 37-0100

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18个已发表图片

35 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

c-Met Antibody in Immunohistochemistry (IHC)

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c-Met Antibody (37-0100) in IHC

Immunohistochemical staining of breast carcinoma tissue using mouse anti-c-Met monoclonal antibody (clone 3D4) (Product # 37-0100).

c-Met Antibody in Immunohistochemistry (Paraffin) (IHC (P))

c-Met Antibody in Western Blot, Immunohistochemistry (WB, IHC)

c-Met Antibody in Western Blot, Immunoprecipitation (WB, IP)

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c-Met Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of C-MET was observed in HeLa which is reported to be positive but not in MCF7 using c-Met Monoclonal Antibody (3D4) (Product # 37-0100) in western blot. {RE}

产品信息

37-0100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化 (IHC)

5-10 µg/mL

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免疫组化（石蜡） (IHC (P))

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

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酶联免疫吸附实验 (ELISA)

0.1-1 µg/mL

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免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

3D4

抗原

Synthetic peptide derived from cytoplasmic domain of human c-Met protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533289

产品详细信息

This antibody is specific for the cytoplasmic domain of the c-Met protein. On western blotting, this antibody recognizes a band at approximately 145 kDa. During development reactivity was confirmed with human HT-29 breast carcinoma lysates and c-Met cytoplasmic domain recombinant protein by both western blot, and with formalin-fixed, paraffin-embedded breast carcinoma tissues by immunohistochemistry.

For immunohistochemistry with formalin-fixed, paraffin-embedded tissues, heat-induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining. Recommended antibody incubation period is overnight at 4°C.

靶标信息

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; Tyrosine-protein kinase Met

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基因别名: AUTS9; c-Met; DFNB97; HGFR; MET; RCCP2

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UniProt ID: (Human) P08581

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Entrez Gene ID: (Human) 4233

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功能

protein tyrosine kinase activity hepatocyte growth factor-activated receptor activity protein binding ATP binding protein phosphatase binding

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