Invitrogen

c-Met Monoclonal Antibody (eBioclone 7), FITC, eBioscience™

Name: c-Met Monoclonal Antibody (eBioclone 7), FITC, eBioscience\u2122
Brand: Invitrogen
SKU: 11-8854-82

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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c-Met Antibody (11-8854-82) in Flow

Mouse c-Met-transfected 293T cells were stained with 0.5 µg of Rat IgG1 K Isotype Control FITC (Product # 11-4301-82) (open histogram) or 0.5 µg of Anti-Mouse c-Met (HGF Receptor) FITC (filled histogram). Total viable cells were used for analysis.

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产品信息

11-8854-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Mouse

已发表种属

C. elegans, Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioclone 7

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_494112

产品详细信息

Description: The eBioclone 7 monoclonal antibody was generated against a mouse c-Met-Ig fusion protein, and reacts with mouse c-Met-transfected cells. Mouse c-Met (HGFR) is a 170 kDa receptor tyrosine kinase (RTK) expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC gamma. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis. Mutations in human c-Met have been implicated in the development of several malignancies.

Applications Reported: This eBioclone 7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioclone 7 antibody has been tested by flow cytometric analysis of mouse c-Met-transfected 293T cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

生物信息学

蛋白别名: cMet; EC 2.7.10.1; hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met proto-oncogene tyrosine kinase; oncogene MET; proto-oncogene c-Met; sc MET; scatter factor receptor; SF receptor; soluble c met; tyrosine-protein kinase Met

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基因别名: AI838057; c-Met; HGF; HGFR; Par4

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Entrez Gene ID: (Mouse) 17295

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功能