Invitrogen

c-Rel Recombinant Rat Monoclonal Antibody (1RELAH5), Alexa Fluor™ Plus 647

Name: c-Rel Recombinant Rat Monoclonal Antibody (1RELAH5), Alexa Fluor\u2122 Plus 647
Brand: Invitrogen
SKU: 740087TP647

高级验证

查看其他26个c-Rel抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)
高级验证 (1)

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c-Rel Antibody (740087TP647) in WB

Western blot was performed using c-Rel Recombinant Rat Monoclonal Antibody (1RELAH5), Alexa Fluor™ Plus 647 (Product # 740087TP647) and ~ 65 kDa band corresponding to c-Rel was observed across cell lines tested. Whole cell extracts (40 µg lysate) of EL4 (Lane 1), RAW 264.7 (Lane 2) and NIH/3T3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot™ 2 Dry Blotting System (Product # IB21001) and blocked w... View More

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c-Rel Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of c-Rel was observed in EL4 and RAW 264.7 cell lysates when compared to NIH/3T3 cell lysate, using c-Rel Recombinant Rat Monoclonal Antibody (1RELAH5), Alexa Fluor™ Plus 647 (Product # 740087TP647) in Western blot. {RE}

产品信息

740087TP647

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

流式细胞分析 (Flow)

0.5 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1RELAH5

偶联物

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

激发/发射光谱

658/675 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

proprietary buffer, pH 6.3-7.3

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

AB_3699432

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain, ' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).

仅用于科研。不用于诊断过程。未经明确授权不得转售。