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Agrisera
cpCK2/Casein kinase II subunit alpha (chloroplastic) Polyclonal Antibody
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cpCK2/Casein kinase II subunit alpha (chloroplastic) Antibody (AS16-3211) in WB
Recombinant cp CK 2 proteins with transit peptide were expressed in DH 5 a cell line by adding IPTG. Lysates were extracted at 4,8 and 16 hrs induction Cells were cultured at RT for 4,8 and 16 hours after IPTG input and were spin-down as indicated time. Cells were washed with cell wash buffer (50 m M MOPS p H 7.5,150 m M Na Cl) and spin-down again. Pellets were resuspended with 5 m L of cell wash buffer and... View More
产品信息
AS16-3211
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CPCK2 protein.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana recombinant cpCK2
靶标信息
cpCK2 encodes a nucleus-encoded, chloroplast-targeted serine/threonine protein kinase that localizes predominantly to the chloroplast stroma, where it functions as a CK2-like catalytic subunit associated with plastid gene expression control. The protein is structurally CK2 alpha-like, comprising a conserved protein-kinase catalytic core (typical bilobal kinase architecture with an ATP-binding site and catalytic residues) and multiple conserved cysteine residues implicated in redox-sensitive regulation, consistent with its proposed role in integrating chloroplast redox state with phosphorylation-dependent signaling. Functionally, cpCK2 (also described in the literature as a plastid transcription kinase activity) phosphorylates plastid transcription machinery components and plastid sigma factors (e.g., SIG1/SIG6), thereby modulating promoter recognition and transcription by the plastid-encoded RNA polymerase system; phosphorylation can differentially influence sigma factor DNA-binding and promoter selectivity in vitro and is implicated in tuning chloroplast transcriptional outputs in vivo. More recently, the chloroplast-localized CK2 alpha subunit CPCK2 has also been shown to act in chloroplast-mediated defense regulation by phosphorylating the salicylic-acid-binding protein SABP3/carbonic anhydrase, promoting its S-nitrosylation and thereby dampening salicylic acid accumulation and innate immune responses, linking cpCK2-dependent phosphorylation to chloroplast redox/post-translational control pathways that impact nuclear-coordinated stress and immunity programs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cpCK2; cpCK2alpha; pCK2; PTK
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