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Invitrogen
degP Polyclonal Antibody
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degP Antibody (PA5-144492) in WB
Western blot analysis of degP in recombinant protein using a dilution of 1:2,000. Samples were incubated with degP polyclonal antibody (Product # PA5-144492). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 62.8 kDa. Observed band size: 65 kDa.
产品信息
PA5-144492
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Escherichia coli Periplasmic serine endoprotease DegP (degP) (27-474AA)
偶联物
形式
Liquid
浓度
5 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% Proclin 300
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
DegP acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures (PubMed:10319814). Degrades transiently denatured and unfolded or misfolded proteins which accumulate in the periplasm following heat shock or other stress conditions (PubMed:16303867). DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids (PubMed:8830688). Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins (PubMed:8830688). Its proteolytic activity is essential for the survival of cells at elevated temperatures (PubMed:7557477). It can degrade IciA, Ada, casein, globin and PapA. DegP shares specificity with DegQ (PubMed:8830688). DegP is also involved in the biogenesis of partially folded outer-membrane proteins (OMP).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: htrA; ptd
