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Agrisera
eEF1B-alpha2/elongation factor 1B-alpha 2 Polyclonal Antibody
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eEF1B-alpha2/elongation factor 1B-alpha 2 Antibody (AS10-679) in WB
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins alpha 1 (3) and alpha 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-Rad). Blots were blocked followi... View More
产品信息
AS10-679
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant eEF1B-alpha2 protein from Arabidopsis thaliana with no affinity tag.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
EF1B2, also called elongation factor 1 beta 2, is a cytosolic subunit of the EF1B complex that serves as a guanine nucleotide exchange factor for EF1A, reloading EF1A with GTP to sustain delivery of aminoacyl tRNAs to ribosomes during translation elongation in plants. EF1B2 partners with EF1B gamma and EF1B delta to form a stable exchange complex, and its activity influences translational throughput, growth, and stress adaptation. Expression of EF1B2 is generally strong in actively dividing and expanding tissues and can shift with environmental stresses, hormone signals, and developmental transitions. Beyond translation, EF1B2 and its complex contribute to cytoskeletal dynamics and vesicle trafficking via EF1A interactions, linking protein synthesis capacity to broader cellular organization. When used as a reference gene, EF1B2 requires validation under specific experimental conditions because its expression can vary with treatments.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 2810017J07Rik; Eef1b; EF-1-beta
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