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Agrisera
eEF1B-beta1 and 2/elongation factor 1-beta1 and 1-beta2 Polyclonal Antibody
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eEF1B-beta1 and 2/elongation factor 1-beta1 and 1-beta2 Antibody (AS10-677) in WB
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins beta 1 (3) and beta 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-Rad). Blots were blocked following... View More
产品信息
AS10-677
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant eEF1B-beta1 protein from Arabidopsis thaliana with no affinity tag.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
EEF1D encodes a ubiquitously expressed subunit of the eukaryotic translation elongation factor 1 complex that localizes predominantly to the cytoplasm, with an alternatively spliced long isoform that can also function in the nucleus during cellular stress. The protein is produced as multiple isoforms: shorter forms that act within the elongation factor 1B (eEF1B) complex and a distinct long form generated by alternative splicing. Structurally, the shorter isoforms comprise the core elongation-factor subunit that associates with other eEF1 components, whereas the long isoform contains additional N-terminal sequence that confers stress-responsive, transcriptional regulatory activity. Functionally, the canonical role of EEF1D is as a guanine nucleotide exchange factor within the eEF1 complex, promoting recycling of eEF1A by facilitating GDP-to-GTP exchange to support delivery of aminoacyl-tRNAs to the ribosome during translation elongation; beyond this housekeeping function, the long isoform has been implicated in activating heat-shock element-containing genes as part of the heat shock response, and EEF1D is a demonstrated physiological substrate of protein kinase CK2, consistent with regulation by phosphorylation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EF-1D; EF1D; FP1047; NEDTCHAL; NY-CO-4
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