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Invitrogen
eGFP Monoclonal Antibody (F56-6A1.2.3)
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eGFP Antibody (MA1-952) in ICC/IF
Immunofluorescent analysis of GFP Tag was performed using H3-GFP construct transfected in HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with eGFP Monoclonal Antibody (F56-6A1.2.3) (Product # MA1-952) at 1:100 dilution and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mous... View More
产品信息
MA1-952
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
C. elegans,
Tag
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
F56-6A1.2.3
抗原
Full length enhanced green fluorescent protein (eGFP)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-952 contains 100 µg of in vitro produced, Protein A purified IgG (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.
MA1-952 detects eGFP and eGFP tagged proteins.
MA1-952 has been successfully used in Western blot and immunofluorescence applications.
The MA1-952 immunogen is full length enhanced green fluorescent protein (eGFP).
靶标信息
eGFP (green fluorescent protein) fluorescent protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
