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Invitrogen
eIF3a Polyclonal Antibody
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eIF3a Antibody (PA5-31296) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of eIF3a was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF3a Polyclonal Antibody (Product # PA5-31296) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
产品信息
PA5-31296
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the N-terminus region of human EIF3A. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.96 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: Raji.
Predicted reactivity: Mouse (99%), Rat (100%), Xenopus laevis (97%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 150/170; centrosomin homolog; cytoplasmic protein p167; eIF-3-theta; eIF3 p167; eIF3 p180; eIF3 p185; EIF3, p180 subunit; EIF3A; Eukaryotic translation initiation factor 3 subunit 10; Eukaryotic translation initiation factor 3 subunit A; eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD); eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD); eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa; eukaryotic translation initiation factor 3, subunit 10, 170kD; eukaryotic translation initiation factor 3, subunit A
基因别名: EIF3; eIF3-p170; eIF3-theta; EIF3A; EIF3S10; KIAA0139; P167; p180; p185; TIF32
UniProt ID: (Human) Q14152
Entrez Gene ID: (Human) 8661
