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Invitrogen
eIF4G3 Polyclonal Antibody
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eIF4G3 Antibody (PA5-89943) in WB
Western blot analysis of eIF4G3 in extracts of various cell lines. Samples were incubated with eIF4G3 Polyclonal antibody (Product # PA5-89943) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-89943
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1200 of human EIF4G3 (NP_0037512)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.74 mg/mL
规格
74 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: HKEAKIEEQE EQRKVQQLMT KEKRRPGVQR VDEGGWNTVQ GAKNSRVLDP SKFLKITKPT IDEKIQLVPK AQLGSWGKGS SGGAKASETD ALRSSASSLN RFSALQPPAP SGSTPSTPVE FDSRRTLTSR GSMGREKNDK PLPSATARPN TFMRGGSSKD LLDNQSQEEQ RREMLETVKQ LTGGVDVERN STEAERNKTR E; Positive Samples: LO2, HeLa
靶标信息
The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: RP11-190H11.1
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