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Invitrogen
eRF1 Monoclonal Antibody (3B6)
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eRF1 Antibody (MA5-49271) in ICC/IF
Immunocytochemistry analysis of eRF1 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in eRF1 Monoclonal antibody (Product # MA5-49271) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for ... View More
产品信息
MA5-49271
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
3B6
抗原
E.coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human Raji whole cell, human HEPG2 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell. IHC: human tonsil tissue, human ovarian cancer tissue, human breast cancer tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, rat kidney tissue. ICC/IF: MCF-7 cell. Flow: HEPA1-6 cell, CACO-2 cell, RH35 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eRF1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; polypeptide chain release factor 1; Protein Cl1; sup45 (yeast omnipotent suppressor 45) homolog-like 1; TB3-1
基因别名: AI463371; D5S1995; D6Ertd109e; ERF; ERF1; ETF1; RF1; SUP45L1; TB3-1
UniProt ID: (Human) P62495, (Mouse) Q8BWY3, (Rat) Q5U2Q7
Entrez Gene ID: (Human) 2107, (Mouse) 225363, (Rat) 307503
