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Invitrogen
eRF1 Monoclonal Antibody (3E5)
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eRF1 Antibody (MA5-49272) in ICC/IF
Immunocytochemistry analysis of eRF1 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in eRF1 Monoclonal antibody (Product # MA5-49272) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
产品信息
MA5-49272
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
3E5
抗原
E.coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human Raji whole cell, human HEPG2 whole cell, human HEPG2 whole cell, rat liver tissue, rat testis tissue, rat kidney tissue, rat PC-12 whole cell, mouse liver tissue, mouse testis tissue, mouse kidney tissue, mouse RAW2647 whole cell. IHC: human tonsil tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, rat pancreas tissue. ICC/IF: A431 cell. Flow: Caco-2 cell, HEPA1-6 cell, RH35 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eRF1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; polypeptide chain release factor 1; Protein Cl1; sup45 (yeast omnipotent suppressor 45) homolog-like 1; TB3-1
基因别名: AI463371; D5S1995; D6Ertd109e; ERF; ERF1; ETF1; RF1; SUP45L1; TB3-1
UniProt ID: (Human) P62495, (Mouse) Q8BWY3, (Rat) Q5U2Q7
Entrez Gene ID: (Human) 2107, (Mouse) 225363, (Rat) 307503
