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Invitrogen
hnRNP H3 Polyclonal Antibody
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hnRNP H3 Antibody (PA5-144017) in ICC/IF
Immunocytochemistry analysis of hnRNP H3 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in hnRNP H3 Polyclonal antibody (Product # PA5-144017) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets approp... View More
产品信息
PA5-144017
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human HNRNPH3 recombinant protein (Position: K29-N268).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human PC-3 whole cell, human Raji whole cell, human K562 whole cell, human Caco-2 whole cell, human MCF-7 whole cell, human HL-60 whole cell, human PC-3 whole cell. IHC: human breast cancer tissue, human gastric cancer lymph node tissue, human liver cancer tissue, human ovarian serous adenocarcinoma tissue, human pancreatic cancer tissue, human placenta tissue, human rectal cancer tissue. ICC/IF: MCF-7 cell. Flow: K562 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
HNRPH3 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein has two repeats of quasi-RRM domains that bind to RNAs. It is localized in nuclear bodies of the nucleus. This protein is involved in the splicing process and it also participates in early heat shock-induced splicing arrest by transiently leaving the hnRNP complexes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Heterogeneous nuclear ribonucleoprotein 2H9; Heterogeneous nuclear ribonucleoprotein H3; heterogeneous nuclear ribonucleoprotein H3 (2H9); hnRNP 2H9; hnRNP H3
基因别名: 2H9; HNRNPH3; HNRPH3
UniProt ID: (Human) P31942
Entrez Gene ID: (Human) 3189
