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Invitrogen
kaiC Polyclonal Antibody
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kaiC Antibody (PA5-144442) in WB
Western blot analysis of kaiC in recombinant protein using a dilution of 1:2,000. Samples were incubated with kaiC polyclonal antibody (Product # PA5-144442). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 56.8 kDa. Observed band size: 69 kDa.
产品信息
PA5-144442
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Nostoc sp. (strain PCC 7120/SAG 25.82/UTEX 2576) Circadian clock protein kinase KaiC (1-270aa)
偶联物
形式
Liquid
浓度
3.69 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% Proclin 300
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
Central component of the KaiABC oscillator complex, which constitutes the main circadian regulator in cyanobacteria. Complex composition changes during the circadian cycle to control KaiC phosphorylation. KaiA stimulates KaiC autophosphorylation, while KaiB sequesters KaiA, leading to KaiC autodephosphorylation. Clock output pathways impact the RpaA transcriptional regulator. KaiC enhances the autophosphorylation activity of SasA, which then transfers its phosphate group to RpaA to activate it. KaiB and KaiC together enhance the phospho-RpaA dephosphatase activity of CikA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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