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mCherry Antibody (740003T) in WB
Western blot was performed using mCherry tag Recombinant Rat Monoclonal Antibody (16D7), Purified (Product # 740003T) by loading whole cell extracts of transiently transfected HEK-293E lysates: H3-mCherry, 5 µg (Lane 1), mCherry-GST, 0.125 µg (Lane 2), mCherry-GST, 0.25 µg (Lane 3), mCherry-GST, 0.5 µg (Lane 4), GST-mCherry, 0.125 µg (Lane 5), GST-mCherry, 0.25 µg (Lane 6), GST-mCherry, 0.5 µg (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitroce... View More
产品信息
740003T
产品规格
种属反应
Chemical
宿主/亚型
Rat
/ IgG2a, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
16D7
抗原
Full-length protein mCherry
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
mCherry Tag recombinant rat monoclonal antibody specifically recognizes mCherry Tag at both C and N-terminus of proteins.
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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