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          • Primary Antibodies ›
          • mTOR Antibodies

          Invitrogen

          mTOR Recombinant Rabbit Monoclonal Antibody (7H13L18)

          2个已发表图片
          3 篇参考文献
          查看其他52个mTOR抗体

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          Cite mTOR Recombinant Rabbit Monoclonal Antibody (7H13L18)

          Additional Information:
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          • 抗体检测数据 (2)
          • 已发表图片 (2)
          mTOR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          mTOR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 4

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          mTOR Antibody (701483) in ICC/IF

          Immunofluorescent analysis of mTOR in HeLa cells using a mTOR recombinant rabbit monoclonal antibody (Product # 701483) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic and nuclear localization of mTOR. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          mTOR Antibody in Immunocytochemistry (ICC/IF)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Recombinant Rabbit Monoclonal Antibody (7H13L18)

          产品信息

          701483

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          2-3 µg/mL
          查看3篇已发表文章 3篇已发表文章

          免疫细胞化学 (ICC/IF)

          1-2 µg/mL
          -
          产品规格

          种属反应

          Human

          已发表种属

          Human

          宿主/亚型

          Rabbit / IgG

          Expression System

          Expi293

          分类

          Recombinant Monoclonal

          类型

          Antibody

          克隆号

          7H13L18

          抗原

          Recombinant protein corresponding to amino acids 1126-1411 of human mTOR
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          Protein A

          保存液

          PBS

          内含物

          0.09% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Wet ice

          RRID

          AB_2532486

          产品详细信息

          Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          靶标信息

          FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR; Tyrosine-protein kinase mTOR; unnamed protein product

          查看更多 收起

          基因别名: FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS

          查看更多 收起

          UniProt ID: (Human) P42345

          查看更多 收起

          Entrez Gene ID: (Human) 2475

          查看更多 收起

          功能
          nucleotide binding inositol hexakisphosphate binding RNA polymerase III type 1 promoter sequence-specific DNA binding RNA polymerase III type 2 promoter sequence-specific DNA binding RNA polymerase III type 3 promoter sequence-specific DNA binding TFIIIC-class transcription factor binding protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity protein binding ATP binding kinase activity transferase activity identical protein binding ribosome binding ion channel binding macromolecular complex binding phosphoprotein binding protein serine kinase activity
          参与通路
          autophagosome assembly regulation of cell growth cytoplasmic translation T-helper 1 cell lineage commitment heart morphogenesis heart valve morphogenesis energy reserve metabolic process 'de novo' pyrimidine nucleobase biosynthetic process regulation of translational initiation ubiquitin-dependent protein catabolic process inflammatory response cellular response to DNA damage stimulus cytoskeleton organization lysosome organization germ cell development positive regulation of cell proliferation insulin receptor signaling pathway regulation of cell size cellular response to starvation response to heat response to virus post-embryonic development positive regulation of biosynthetic process regulation of autophagy negative regulation of autophagy positive regulation of autophagy positive regulation of lamellipodium assembly positive regulation of epithelial to mesenchymal transition positive regulation of myotube differentiation macroautophagy regulation of macroautophagy negative regulation of macroautophagy regulation of lipid metabolic process neuronal action potential cell projection organization positive regulation of cell growth positive regulation of actin filament polymerization SCF-dependent proteasomal ubiquitin-dependent protein catabolic process T cell costimulation ruffle organization regulation of myelination protein destabilization response to nutrient levels cellular response to nutrient levels cellular response to nutrient TOR signaling response to insulin cellular response to insulin stimulus regulation of actin cytoskeleton organization calcineurin-NFAT signaling cascade cellular response to amino acid starvation multicellular organism growth TORC1 signaling TORC2 signaling regulation of circadian rhythm negative regulation of apoptotic process response to amino acid anoikis protein kinase B signaling positive regulation of cell differentiation regulation of osteoclast differentiation positive regulation of translation negative regulation of cell size positive regulation of glycolytic process negative regulation of innate immune response positive regulation of transcription from RNA polymerase III promoter negative regulation of translational initiation positive regulation of translational initiation negative regulation of insulin receptor signaling pathway positive regulation of lipid biosynthetic process behavioral response to pain oligodendrocyte differentiation positive regulation of oligodendrocyte differentiation cardiac muscle tissue development protein stabilization voluntary musculoskeletal movement positive regulation of multicellular organismal process positive regulation of stress fiber assembly positive regulation of keratinocyte migration regulation of protein kinase B signaling positive regulation of protein kinase B signaling cardiac cell development cardiac muscle cell development cardiac muscle contraction cellular response to methionine positive regulation of SCF-dependent proteasomal ubiquitin-dependent catabolic process negative regulation of calcineurin-NFAT signaling cascade cellular response to amino acid stimulus cellular response to leucine cellular response to hypoxia cellular response to osmotic stress regulation of cellular response to stress regulation of membrane permeability vascular endothelial cell response to laminar fluid shear stress antibacterial innate immune response regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of wound healing, spreading of epidermal cells negative regulation of TORC2 signaling regulation of locomotor rhythm negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of cytoplasmic translational initiation regulation of lysosome organization negative regulation of lysosome organization positive regulation of pentose-phosphate shunt cellular response to leucine starvation negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of cytoplasmic translation regulation of autophagosome assembly
          It has to be done as per old AB suggested Products section.

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