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Invitrogen
mtHSP70 Monoclonal Antibody (JG1)
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.jpg?time=20240507?time=20240507 "mtHSP70 Antibody (MA3-028) in WB")
.jpg?time=20240507?time=20240507 "mtHSP70 Antibody (MA3-028) in WB")
产品信息
MA3-028
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human,
Mouse,
Non-human primate
已发表种属
C. elegans,
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Mouse
/ IgG3
分类
Monoclonal
类型
Antibody
克隆号
JG1
抗原
Synthetic peptide corresponding to residues G(615) S G S S G T G E Q K E D Q K E E K Q(633) of mouse mtHSP70.
偶联物
形式
Liquid
浓度
1.07 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-028 detects mitochondrial heat shock protein 70 kDa (mtHSP70) from human, non-human primate, canine and mouse tissues.
MA3-028 has been successfully used in Western blot, immunocytochemical, immunofluorescence, immunohistochemical (paraffin), and immunoprecipitation procedures. By Western blot, this antibody detects a single ~75 kDa band representing mtHSP70 from U2OS cell homogenate. Immunocytochemical staining of mtHSP70 in DAP.3 cells with MA3-028 results in a worm-like staining pattern, consistent with mitochondrial localization.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
靶标信息
The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein which is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP70 (mtHSP70, GRP75 or mortalin) a 75 kDa protein that is found within the mitochondria. mtHSP70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP70 and other inner membrane proteins of the mitochondria.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 68 kDa heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; Heat shock protein family A member 1A; Heat shock protein family A member 1B; heat shock protein, 70 kDa 3; heat shock-induced protein; Hsp68; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70.1/HSP70.2; HSP70.3; inducible heat shock protein 70
基因别名: HEL-S-103; hsp68; HSP70-1; HSP70-1A; Hsp70-3; HSP70.1; Hsp70.3; Hsp70A1; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSX70
UniProt ID: (Human) P0DMV8, (Mouse) Q61696
Entrez Gene ID: (Human) 3303, (Mouse) 193740
virus receptor activity
G-protein coupled receptor binding
receptor binding
protein binding
ATP binding
ATPase activity
enzyme binding
heat shock protein binding
ubiquitin protein ligase binding
ATPase activity, coupled
histone deacetylase binding
protein binding involved in protein folding
unfolded protein binding
C3HC4-type RING finger domain binding
cadherin binding involved in cell-cell adhesion
protease binding
double-stranded RNA binding
poly(A) RNA binding
protein N-terminus binding
NF-kappaB binding
telomere maintenance
DNA repair
protein folding
response to heat
positive regulation of gene expression
regulation of cell death
regulation of protein ubiquitination
negative regulation of protein ubiquitination
positive regulation of interleukin-8 production
cellular response to oxidative stress
cellular response to heat
protein refolding
regulation of mRNA stability
ATP metabolic process
viral entry into host cell
protein stabilization
positive regulation of NF-kappaB transcription factor activity
negative regulation of cell death
cellular heat acclimation
positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway
negative regulation of inclusion body assembly
cell-cell adhesion
regulation of cellular response to heat
negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of endoribonuclease activity
positive regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of mRNA endonucleolytic cleavage involved in unfolded protein response
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
