Invitrogen

nNOS Polyclonal Antibody

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95 篇参考文献
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说明书
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nNOS Polyclonal Antibody

产品信息

61-7000

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1 µg/mL
查看14篇已发表文章 14篇已发表文章

免疫组化 (IHC)

-
查看56篇已发表文章 56篇已发表文章

免疫组化（石蜡） (IHC (P))

5-10 µg/mL
查看6篇已发表文章 6篇已发表文章

免疫组化（冰冻） (IHC (F))

1-2 µg/mL
查看3篇已发表文章 3篇已发表文章

免疫组织化学（悬浮细胞） (IHC (Free))

-
查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

-
查看9篇已发表文章 9篇已发表文章

酶联免疫吸附实验 (ELISA)

0.1-1 µg/mL
-

免疫沉淀 (IP)

-
查看2篇已发表文章 2篇已发表文章

免疫电镜技术 (IM)

-
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产品规格

种属反应

Mouse, Rat

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A recombinant protein consisting of 195 amino acids from the N-terminus of rat nNOS protein.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2313734

产品详细信息

This antibody reacts with the ~160 kDa nNOS protein and does not exhibit any cross-reactivity with the related eNOS or iNOS proteins. During development reactivity was confirmed with a ~160 kD band on western blots of rat and mouse brain tissue lysates (20 µg). For use in immunohistochemistry with paraffin embedded tissues some background staining was evident.

靶标信息

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS

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基因别名: 2310005C01Rik; Bnos; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; Nos1

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UniProt ID: (Rat) P29476, (Mouse) Q9Z0J4

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Entrez Gene ID: (Rat) 24598, (Mouse) 18125

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功能
NADPH-hemoprotein reductase activity nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding amino acid binding sodium channel regulator activity enzyme binding heme binding protein homodimerization activity ion channel binding cadmium ion binding flavin adenine dinucleotide binding NADP binding ATPase binding scaffold protein binding oxidoreductase
参与通路
response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process muscle contraction striated muscle contraction nitric oxide mediated signal transduction female pregnancy aging regulation of heart contraction negative regulation of cell proliferation response to heat response to organonitrogen compound response to lead ion response to organic cyclic compound response to activity peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to nutrient levels response to lipopolysaccharide negative regulation of peptidyl-serine phosphorylation response to vitamin E multicellular organismal response to stress positive regulation of histone acetylation response to nicotine exogenous drug catabolic process negative regulation of apoptotic process negative regulation of potassium ion transport response to peptide hormone response to estrogen response to ethanol negative regulation of blood pressure negative regulation of heart contraction positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of sensory perception of pain oxidation-reduction process cellular response to mechanical stimulus cellular response to growth factor stimulus response to nitric oxide cellular response to epinephrine stimulus positive regulation of the force of heart contraction positive regulation of long-term synaptic potentiation positive regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of neuron death positive regulation of sodium ion transmembrane transport
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